LOCUS       40924_12770        5296 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gateway recycling vector pCON-R5R6 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Kimura T, Shibahara K, Tanaka Y, Nakao A, Kawazu T, Nakagawa T.
  TITLE     Gateway Recycling Cloning System for Multigene Construction
  JOURNAL   Published Only in Database (2012)
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Kimura T, Shibahara K, Tanaka Y, Nakao A, Kawazu T, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2012) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 5296)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5296)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Published 
            Only in Database (2012)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-OCT-2012) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pDONR201.
FEATURES             Location/Qualifiers
     source          1..5296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    683..806
                     /label=attR5
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(910..1566)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     misc_feature    2234..2257
                     /label=rare cutter sites
                     /note="rare cutter sites"
     protein_bind    complement(2524..2647)
                     /label=attR6
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3169..3268)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3391..4197
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      4343..4931
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(5066..5093)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(5185..5271)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtatagaaaa
      121 gtaggctgga tgaatcgaat aacttcttct tcaaatgcgc aaacgaagaa atttcctcag
      181 tctcagccac agccgccgga gaaaaatgag aaaaaactga aaagggagag atttccggtg
      241 aaccctttga gagatttcag tacaagaagc tctggaagca gcagttgtag caatgagagt
      301 gcttctggtt caacttccgg tgaagcatcc aacggttgtc acaggttctt gctctctcat
      361 tctttctcat cgtcttcttc atcatcttct tcttcgttgg gagtttttcc tcgtagacca
      421 gtgagatcag tagctaaaaa ccccaaatct gctccggtag ttagtaaacc cttaattaga
      481 aagaaaccaa gctctcttga ggaggttaag ttgaaatcta ctcttactga gaaacctaat
      541 ttgctgaagt ctcagagatg caagacaaat cctgtatctg gaaagagacc aacttgtaag
      601 attaccatga aaccggagaa agtttcggtt ttgaagaaac aatcttctgt ttctagactg
      661 tggccgcgtg gatcccccat cacaactttg tatacaaaag ttgaacgaga aacgtaaaat
      721 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt
      781 aaaacacaac atatccagtc actatggcgg ccacatttac atatcagtat atattcttat
      841 accgcaaaaa tcagcgcgca aatacgcata ctgttatctg gcttttagta agccggatcc
      901 acgcgattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
      961 gccgacatgg aagccatcac agacggcatg atgaacctga atcgccagcg gcatcagcac
     1021 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     1081 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     1141 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     1201 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     1261 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     1321 cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag
     1381 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     1441 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     1501 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     1561 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     1621 tgatcttatt tcattatggt gaaagttgga acctcttaaa atgcatgggc aaatattata
     1681 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtctgtgatg
     1741 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
     1801 gggcgtaaac gcgtggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg
     1861 ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag
     1921 gtgtgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa
     1981 ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt
     2041 cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg
     2101 tttattgaaa tgaacggctc ttttgctgac gagaacaggg actggtgaaa tgcagtttaa
     2161 ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat
     2221 tattgacacg cccatttaaa tgcgatcgct taattaaggg cgacggatgg tgatccccct
     2281 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
     2341 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
     2401 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
     2461 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgatc
     2521 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     2581 atctaattta atatattgat atttatatca ttttacgttt ctcgttcaac tttttaatac
     2641 aaagttgtga tatcaagctg gcgcgcctgt gatcaagctt cgacaattat tttggactct
     2701 catgttctca agctgaaatc caaaagaaac atgatgttgg ttcagatttg gaatcactga
     2761 caatgaagtt tcagtcagcc ggtttgtcac caaggatcca ggcttcatca tgggagccga
     2821 gccgttcaag tttcaacttc gactatttgg ccacatcttc tgattctatt gatctctcac
     2881 agtttcagag aggtttagtt gacaggagtt cttgtcatcc tcatgtaaca ctggataaag
     2941 tgtcaaggac acatttgaga gtagagcaaa caaatagcca catgcctgaa atgaaatccc
     3001 aacagattac agatactgaa tttgatattc aaaatcacaa agaaagtgct gcttctctgg
     3061 gaaaaggaag agaattgctt ccatgctctg ctgcagagtc aataagcact gatggaggtg
     3121 gtctgatttg ttctgaggat tcaaattgga tggcatgtta caagaattac ccaactttat
     3181 tatacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     3241 ctatcagtca aaataaaatc attatttgcc atccagctgc agctctggcc cgtgtctcaa
     3301 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     3361 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     3421 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     3481 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     3541 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     3601 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     3661 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     3721 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     3781 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     3841 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     3901 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     3961 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     4021 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     4081 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     4141 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     4201 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     4261 gcaagctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4321 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4381 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4441 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     4501 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4561 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4621 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4681 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4741 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4801 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4861 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4921 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4981 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcta
     5041 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     5101 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     5161 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     5221 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
     5281 agttccctac tctcgc
//
